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Epilepsy is the fourth leading cause of death in the US, despite the availability of anti-seizure drugs. The lack of therapeutic options for patients with medically refractory epilepsy, who remain on two or more anti-seizure drugs and have failed multiple seizure-control trials, can be discouraging for the patient, his or her family, and the health care team. Technological advancements in the understanding of epilepsy and the development of more effective anti-seizure drugs have resulted in the use of various combinations of anti-seizure drugs. Other medications that affect brain function have also been used to treat patients with intractable epilepsy. These drugs fall into two broad categories: those that act by augmenting and modulating the action of neurotransmitters and those that act at the cellular level by inducing apoptosis. These drugs are usually used in the context of an interdisciplinary treatment approach that includes psychosocial issues, seizure diaries, cognitive assessment, and monitoring of the patient’s seizure activity. This article reviews these strategies as they are currently being implemented in the care of patients with medically refractory epilepsy.Nucleotide-protein interactions in the promoter region of Escherichia coli K-12 lacZ gene.
E. coli K-12 cells were analysed for the presence of regulatory RNA species which may be transcribed from the upstream regions of the lacZ gene. The operator sequence of the lac-operon, located at -38 nucleotides from the initiation site, was shown to be the sole binding site for the protein LacI. DNase I footprinting of the gene’s promoter region suggested that LacI binds to two
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